high quality germany antioxidant dtpd ( dmpd)
high quality germany antioxidant dtpd ( dmpd)
high quality germany antioxidant dtpd ( dmpd)
high quality germany antioxidant dtpd ( dmpd)
high quality germany antioxidant dtpd ( dmpd)
  • What does DMPD stand for?
  • N,N -Dimethyl- p -phenylenediamine dihydrochloride (DMPD) 1 is a compound that is normally used to measure the antioxidant potentials of fruit juices and other natural products. In the presence of ferric iron, it gets converted to DMPD + radicals, which are scavenged by antioxidant molecules present in test samples .
  • What is the absorbance peak of DMPD +?
  • The DMPD ·+ radical shows an absorbance peak at 505 nm (Sripakdee et al. 2015 ). Radical DMPD ·+ was generated from the mixture of 1 mL of 100 mM DMPD, 100 mL of 0.1 M acetate buffer pH 5.25 and 0.4 mL of 0.05 M FeCl 3.
  • How to prepare DMPD acetate buffer?
  • Stock solution of DMPD (100 mM) was prepared by dissolving 209 mg of DMPD in 10 ml of water. Acetate buffer (100 mM, pH 5.25) was prepared by proportionate mixing of 100 mM sodium acetate (75.98 ml) and 100 mM acetic acid (24.02 ml) and bringing the pH to 5.25.
  • What happens if DMPD is mixed with ferric iron?
  • Normally, excess of DMPD with limiting concentration of ferric iron brings a reproducible result. If the solution is mixed with any antioxidant molecules (e.g., Trolox, ascorbic acid), the Fe (III) iron gets converted to Fe (II) iron and the color gets reduced.